What is tissue engineering?
Cells from biopsy are invitro cultivated and expanded
Harvest material is then applied onto a scaffold to form the tissue
Tissue is then implemented
Three pillars: Cells, scaffold, cultivation systems
Name three types of cells
Primary cells: Mature cells with limited potential for differentiation
Cell lines: Mutated or gen-modified primary cells
Stem cells: Undifferentiated cells
Totipotent, Pluripotent, multipotent
Scaffold
Surface for cell attachments
Extracellular matrix (ECM)
Dynamic fibrillar tissue specific network of extracellular macromolecules
Contains of collagen, elastin, fibronectin & laminin, proteoglycans
Name requirements for synthetic scaffolds
Material: Biocompatibility, biodegradeability, sterilizable
Surface: Positive cell-material interaction, wettability
Dimensional architecture: Nutrient supply, tissue-specific size and shape, facilitate cell seeding
Name possible biomaterials
metals
ceramics
carbons
polymers
3D bioprinting types
Extrusion printing
Inkjet printing (thermal of pierzoelectric)
Laser printing
Stereolithography
Example for bioinks
Alginate
Collagen
Gelatin
Requirements for cultivation systems
Nutrient and oxygen supply
Biocompatibility
Mechanical stimulation
Sterilizable
Removal of metabolics
sterile culture environment
Fabrictaion methods for spheroid produtction
3D bioprinting
Hanging drop method
Ultra-low attachment well plates
Three different lyers of spheroids
Proliferation
Nercrosis
Quiesence
Last changed2 years ago