intro to myco 1

– study and identification of the pathogenic yeasts and molds, collectively called FUNGI

– unicellar fungi that reproduce by budding

– multicellular, higher forms of fungi.

MOLDS are composed of filaments called _____. Specialized structures for reproduction arise from the hyphae and produce _____

_____ preparation, illustrating dissection of the colony fragment with needles in a drop of lactophenol aniline blue prior to placement of the coverslip.

-rarely used

-the loop or hook is used to dig out mycelium that is submerged, so as to demonstrate structures produced in this area, such as aleuroconidia.

-the sticky side of tape being pressed to the surface ofthe fungus colony.

-Transparency tape preparation: stretching the inoculated tape over a drop of lactophenol aniline blue on the surface of a microscope slide

-recommended in instances wherein neither the tease mount nor the transparency tape preparation establishes an accurate identification, or when permanent slide mounts are desired for further study or for study use by students. Although somewhat tedious to perform, high-

quality preparations in which the spore structures and arrangements are beautifully preserved can be made.

METHODS/STAINS:

C. neoformans in CSF

detection if all fungi including p. jiroveci

examination of respiratory specimens in p. jiroveci

-melanin stain for histologic sections

- distinguishes c. neoformans from other yeast like candida spp.

Most fungi stain Gram _______ but some,

such as _________, exhibit stippling or appear Gram negative

-General-purpose histologic stain

-detection of dematicious yeast

-Detection of encapsulated yeasts

-insensitive (40%) means of detecting C. neoformans in CSF

-Clearing specimens of cellular debris to make fungi more visible

-Most useful when combined with Calcofluor white