Was sind 2 Probleme der CRISPR/Cas systeme?
Welche Ansätze gibt es umd das System zu verbessern?
Warum ist cas off-target activity a problem?
alternate Gene delivery methods
cas9 nickases
cas9-Fokl Fusion
high-fidelity spCas9 variants
altered gRNA design
switchable cas9
cas9 homologs
Anti-CRISPR Systems
was sind non-canonical?
unit synopsis
revisit cleavage-deficient cas9 and cas9 nickases
CRISPRi
transcriptional regulation in eukaryotes
site specific epigenetic modifikations
site specific base editing
chromatin imaging
chromatin immunoprecipitation (ChIP)
EvolvR for focused random mutagenesis
cas13
Cas13 applications
collateral activity of cas13
SHERLOCK a cas13 application
DETECTR, Nucleic Acid Detection with cas12
SHERLOCK to Detect SARD-CoV-2
Last changed21 days ago
