what was created out of the chloroplasts during the primary endosymbiosis?
plastid-less eukaryote + cyanobacterium
Glaucopyhta
Rhodophyta
Viridiplantae
what is known about cyanobacteria?
gram negative bacteria
O2-evolving photosynthesis
tyhlakoids with PSII, PSI, Cyt b6f
how does the molecular evolution of plastids look like after the primary endosymbiosis?
1st endosymbiosis
what was created out of the chloroplasts during the secondary endosymbiosis?
how does the molecular evolution of plastids look like after the second endosymbiosis?
what does the complex plastids of the seocndary endosymbiosis look like?
which groups are biotechnically interesting?
Diatoms: PUFA
Dinophytes / Dinophytes : toxins, bio-luminescnece
which groups are ecologically important?
50% of the primary production is due to marine ecosystems
basis of the food web
dinophytes as symbionts in corals
how does the molecular evolution of plastids look like after the third endosymbiosis?
how does the evolution of chlorophylls look like?
what makes the thylakoid membrane different?
antenna proteins
how is Chla built?
how is the LHC built and where is it present?
constists of 12 carotenoids:
6 luteins
3 neoxanthins
3 violaxanthins
42 chlorophylls
24 Chl a
18 Chl b
How is the Chl a/c LHC built?
how can you distribute the LHC complexes?
which proteins of the reaction centre are present in PSII?
which proteins are present in the oxygen evolving complex?
what is the different structure of thylakoids?
what are diatoms?
oligomeric states of FCP (free pool and those around PSII) are extremely divers depending on species
what is needed for succesful endosymbiosis?
no digestion of symbiont
gene transfer from endosymbiont to host
regulation between compartments
transporters for exchange between compartments
what are the three ways of transport in complex plastids?
Ribosome of the cytosol > PPC
Ribosomes of the cytosol > plastid
Ribosomes in the PPC > plastid
what do you need to detect translocation?
additonal signal peptide
signal peptide is known (ends with ASA-F motive)
for transit peptides there is only week consenus with plant sequences (no prediction possible), but STTLS is found on position -6 to -1
how do you import proteins into ditaom chlorplasts?
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