ImmunoFEDs are often constructed via functionalization of the gate-insulator surface with antibodies as receptors for the recognition of specific antigens.
Yes
One major problem by biomarker detection in real samples with FETs is the screening of the charge of biomolecules by mobile counter ions in high ionic strength samples.
One major problem by biomarker detection in real samples with FETs is the screening of the charge of biomolecules by mobile counter ions in low ionic strength samples.
No
One major problem by biomarker detection in real samples with FETs is the possible non-specific adsorption of other charged proteins.
Typical insulator thicknesses for ISFETs are about 500 nm.
-> ≤ 100nm
The response of EnFETs can be negatively influenced by the stirring of the analyte.
-> no problem for potentiometry, just bad in amperometry
The response of EnFETs can be negatively influenced by the buffer capacity, ionic strength, pH of analyte, enzyme immobilization method, temperature.
The response of EnFETs can be negatively influenced by the buffer capacity, ionic strength, pH of analyte, enzyme immobilization method, additional salt concentration.
-> salt concentration correlates with ionic strength
The response of EnFETs can be negatively influenced by the thickness of Debye length.
-> H+ ions are always inside the Debye length because they are so small
-> macromolecules would be influenced by the Debye length
The response of EnFETs can be negatively influenced by the measurement mode.
-> measurement mode is just the way how the responds is read
The response of EnFETs can be negatively influenced by the immobilized amount of enzyme units.
-> if there are not enough enzyme units the reaction cannot occur
The response of EnFETs can be negatively influenced when too high substrate concentrations are inside the analyte.
-> too high substrate concentrations lead to self inhibation
A high order ISFET sensor module consists of the same number of measurands as sensors.
-> more measurands than sensors
A high order ISFET sensor module has a higher number of sensors as measurands.
A high order ISFET sensor module utilizes different sensor arrangements and / or same operation modes.
A high order ISFET sensor module utilizes different sensor arrangements and / or different operation modes
A high order ISFET sensor module should have different chemical and physical sensors for chemical and physical measurands.
-> chemical sensors also serve as physical sensors
A high order ISFET sensor module allows differential measurements.
A high order ISFET sensor module is operated at different working points for the particular sensors.
A high order ISFET sensor module can be applied both in stirred and quiescent solutions.
LAPS is a capacitive field-effect sensor, where the light intensity depends on the analyte concentration.
LAPS allows the seperation of several sensor spots by simply addressing them via a modulated light pointer.
LAPS can differentiate between various ions at the same time on the same sensor surface.
LAPS generates a transient current affected by electron / hole pairs and the analyte concentration.
LAPS generates a transient current affected by electron / hole pairs; the analyte concentration has no influence.
The LAPS can be operated in the constant current mode and in the constant phase mode.
The resulting photocurrent in LAPS depends on the wavelength.
The thinner the LAPS chip, the higher is the spatial resolution for LAPS measurements.
-> the thinner the sensor, the less the light beam is scattered
LAPS also suffer from some leakage current due to the diffusion of charge carriers into non-illuminated regions.
LAPS detect the variation of the depletion layer thickness depending on the concentration of the target analyte.
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