With which two approaches can you design new functional proteins?
Which motives are known to work well in bottom-up approaches of protein design?
Describe the geometry of a coiled-coil motive.
Name an application of coiled-coil structures in nature and synthetic biology.
two (or more) alpha-helices interacting with one another
inner AS (blue) are either ionic or hydrophobic so that they can interact well with each other
must have different properties than red aminos acids to prevent interference
Applications:
coiled coil Zn hydrolase
Describe the structure of collagen.
Strand of repeating patterns of three amino acids (X-Y-Gly)n
X: proline (stiffness)
endo- or exo-conformation
Y: Hydroproline (?)
only exo-conformation
necessary for H-bonds
increases stability of collagen
Gly: necessary to provide necessary flexibility (only proline would result in too high strain)
single strands are left-handed
aggregates of 3 single-strands form right-handed superhelix (stabilized by H-bonds perpendicular to helix axis)
Zuletzt geändertvor 9 Monaten
