How can a C-terminal thioester be introduced? Show the reaction scheme for one example using chemical structures.
Show one work-around the use of cysteine at the ligation site (still yielding a native peptide bond) using a reaction scheme and chemical structures.
Explain three methods of protein labeling.
Name two challenges in site specific protein labelling and suggest a potential solution.
Which two amino acids are most frequently used to attach fluorophores? Draw the chemical structure of one labeling agent each (reactive handle + symbol for the fluorophore) and draw the mechanism of attachment.
What is the “click reaction"? Why is this labeling method so efficient? What is the mechanism? (Literature research)
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