1. Introduction and History of Genomics

Which statemen regarding human genomics is correct?

1) Bringing a new drug to the market costs pharmaceutical companies between 1 and 5 million dollars

2) The term genomics was coined 1987 when a new journal with that named was launched

3) The human genome project lasted from 1990 till 2003

4) The recently pusblished Cancer Genome Atlas had data on ~1100 patients

5) The global sequencing market is predicted to reach over 20 billion US$ per year in 2025

What is the size of the human genome?

1) 6.4 Mbp

2) 32 Gbp

3) 3.2 Gbp

4) 6.4 Gbp

5) 3.2 Mbp

Which of these dates does NOT fit the event?

1) 1944 Averys experiments show that DNA is the genetic material

2) 1953 James Watson and Francis Crick discover he double helix structure of DNA

3) 1961 Marshall Nirenberg cracks the genetic code for protein synthesis

4) 1869 Friedrich Miescher identifies “nuclein”

5) 1973 Invention of polymerase chain reaction (PCR)

Which statements regarding genetic maps are correct?

1) Experiments in Drosophila confirmed Mendel’s second law, which states that traits that are on the same chromosomes are inherited in linkage

2) In Drosophila it was first shown that two Mendelian traits segregate in the F2 generations in a 9:3:3:1 ratio

3) In 1913 the first human genetic map was published using DNA-based genetic markers

4) The genetic distance between two loci is measured in centimorgans and is higher the higher the recombination frequency between these two loci is

5) When two traits are linked, their recombination frequency is expected to be below 50%

Males heterozygous for grey body (Gg) and normal wings (Aa) are crossed with females with black body (gg) and krippled wings (aa) that results in the following offspring: 236 grey body, normal wings 253 black body, krippled wings 50 grey body, krippled wings 61 black body, normal wings.

Which statements are correct?

1) There are more recombinant offspring than non-recombinant

2) Grey body is dominant over black body

3) If the gene for body colour and wingshape would be on two different chromosomes, one would expect approx. 150 flies per combination

4) The two studied genes are unlinked

5) The two studied genes are linked with a distance of 50cM

6) The two studied genes are linked with a distance of ~18,5cM

Which statements are correct? Human disease genes can be mapped by positional cloning, because…

1) Traits are inherited independently (Mendels second law)

2) The human genome was sequenced

3) Linkage between polymorphic DNA markers and the disease can be determined in families

4) PCR allowed the generation of “Sequence tagged site”(STS) markers

5) A genetic map of non-polymorphic DNA markers has been developed