5. Other Sequencing Technologies

Which statements regarding these Next-Gen Sequencing technologies are correct?

1) Io Torrent uses emulsion PCR to amplify DNA

2) Ion Torrent has a higher throughput per run than Illumina, but much shorter read lengths

3) Complete Genomics amplifies DNA by rolling circle amplification, resulting in DNA “Nanoballs”

4) The DNBSEQ-T7 is a sequencer based on the Complete Genomics Technology and is currently the biggest competitor for Illumina’s NovaSeq platform

5) BGI announced that their new sequencer will allow to sequence whole human genomes for 100$

Which statements regarding long read technologies are correct?

1) A problem of NGS technologies is that e.g. paired-end sequencing of BAC clones is not possible -> correct, because the NGS libraries need short insert sizes. BAC inserts are too big

2) Mate-pair libraries increase the physical coverage of a genome assembly

3) Synthetic reads refers to improving genome assemblies by synthesizing long DNA molecules

4) Optical Mapping is a NGS sequencing technology

5) Genome conformation mapping does not work from purified DNA

Which statements regarding the PacBio sequencing ttechnology is correct?

1) PacBio detects pH changes to sequence single molecules

2) PacBio uses very small wells to detect the fluorescence in only a very small volume

3) As the fluorophore is attached to the phosphate group i(t)s gets cleaved off when the nucleotide is incorporated

4) The nucleotides that are used for PacBio sequencing do not contain a block at the 3’OH group of the ribose

5) Circular sequencing in PacBio is used to uncrease the sequencing quality per sequenced molecule

f sequencing errors are fully unbiased (=random) and the quality score of single coverage is 10, what is the theoretically expected quality score for 2x coverage?

1) 10

2) 11

3) 20

4) 100

5) Cannot be calculated

 Which statements regarding Oxford Nanopore sequencing are correct?

1) Molecules are imaged by a sensitive nanocamera as they pass through a pore inserted in a membrane

2) An advantage of the technology is that it has the higher accuracy (= highest Phred score) per base sequenced

3) Bases are sequenced by a characteristic change in the current as the base passes through a nanopore

4) Oxford Nanopore can generate sequence reads of over >100kbp

5) Oxford Nanopore sequencers are standard for de novo genome sequencing

Which statement regarding sequencing technologies is correct?

1) BGI sequencing (per Gbp sequenced) is currently cheaper than Illumina sequencing

2) Oxford Nanopore generates long DNA reads but is currently ~1000-fold more expensive (per Gbp sequenced) than Illumina (NovaSeq)

3) The NovaSeq (Illumina) is more expensive (per Gbp sequenced), but more accurate than the Sequel PacBio sequencer

4) The market of NGS is expected to be 1 trillion in 2021

5) Due to the long reads, PacBio currently has >75% of the NGS market