Name label-free imaging tecniques
second harmonic generation
third harmonic generation
What are the differences between second and third harmonic generation?
SHG
second-order non-linear -> w = 2wi
collagen, actomyosin, microtubules, cancer research
strictly requires non-centrosymmetry
THG
third-order non-linear -> w = 3wi
lipid–water interfaces, membranes, organelle boundaries, liquid flow
no requirement for non-centrosymmetry
optimal for 3D imaging
What is MSOT?
MultiSpectral Optoacoustic Tomography
detect acoustic emission, instead of light emission
light absorption -> temperature rise -> thermoelastic expansion -> acoustic emission
acoustic signal need to be mapped to light excitation
How can transducers be arranged in MSOT?
parallel or circular
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