Why are D-peptides interesting for pharmaceutical applications?
Why is the genetic code degenerate (= several base triplets for the same amino acid)?
Degeneracy results because there are more codons than encodable amino acids.
What factors can influence the protein production in E. coli?
-Temperature
-Transcription factors
-inducible Promotor
-salt concentration
Why are protein containers usually highly symmetrical?
smaller repetitive proteins are more easily encoded in DNA
What do you need to know about a reaction to design an enzyme?
Type of reaction? Catalystic elements? Groups/Parts of the molecules that are changed
What is the difference between the design of supercharged GFP and the design of the surface-charged antibody?
-Supercharged GFP: define exposed residues via number of neighbouring atoms
-Surface-charged antibodies: Definition via reference value
Why are there no glide/mirror planes or rotoinversion centres/elements in protein crystal?
Proteins are chiral and consist of Amino acids and mirror planes would convert them into D-Amino acids, which do not exist in nature
What is the size of the asymmetric unit for space group P1? And for space group P-1?
-P1: asymmetric unit = whole unit cell (no symmetry)
-P-1: asymmetric unit = ½ of unit cell (inversion centre => 2 fold redundancy)
Why is it important to test several hundred conditions for the crystallization?
Experimentally screen under which conditions crystallization happens
remarkable resistance to proteolytic degradation compared to their natural L-peptide counterparts
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