What does EPR stand for in a Pharmalogial context
Enhanced Permiability and Retention —> Drugtargeting to target Tumor cells, done with Macromolecules, because they can diffuse in the bad vascular system of Tumors.
What does EPR stand for in an Emergency context?
Emergency Preservation and Resuscitation —> Describes inducing a Hypothermia (cooling down) to the patient to stop/ slow down cell death, down with saline solution, gives the surgeons roughly an hour to stitch up the wound, refill the patient with blood and reheat
Cooling has to be rapid, warm up will be slower
Which detergents can be used for decellularization?
Polyethylene glycol - PEG
Triton-X
Sodium dodecyl sulfate - SDS
Name four traditional TE methods?
Cell Seeding
Cell Moulding/ Casting
Sheet Rolling
In-Situ TE
Bed type
Inexpensive
Very common
moving the object
Console Type
fast lateral movement
compact
Gantry Type
Solid bed
more equipment (2nd mounting)
requires sturdy design
Which of the following substances are vasoconstrictors?
Which of the following substances are vasodilators?
What is understood as Wettability, and how does it relate with surface tension?
Wettability describes, how a surface-liquid combination will interact. A high surface tension of the liquid results in a bad wettability:
What is important for good Tissue integration? (Immune Reaction)
And how can it be guided?
Early recruitment of Mesocymal Stemm Cells
TNF-α inhibition (Unterdrücken/ Hemmen)
IL4 & IL13 Secretion
Trophic factor secretion
—> M2/ Th2 dominant response —> Funcitonal Regeneration
Reaction can be guided by adding suitable chemokines.
What is bad regarding Tissue integration? (Immune Reaction)
TNF-α secretion (Ausschüttung)
Interferon-γ secretion (gamma)
—> M1/ Th1 dominant response —> Fibrosis
Name the three phases of the host response after implantation?
Inlammation
Proliferation
Remodeling
Ordne OH zu
Ordne COOH zu
Ordne NH2 zu
Ordne CONH2 zu
Name the 4(4.5) Process steps in traditional TE:
Same the Scaffold fabrication methods:
Nenne ein bsp. für ein Markerpaar, mit dem ein Live/ Dead Assay stattfinden kann.
FDA (Fluorescein —> Live, green)/ PI (dead, red)
What is understood as a Cimino avf?
A Cimino fistula (avf: arteriovenous fistula)
It to support dialysis
Hydrogels are a fundamental component of bioinks. In the context ot the bioprinting process, different requirements are demanded of this class of materials. Which parameters can be varied to adjust the viscosity, gelation kinetics and network properties of a hydrogel.
Exam Question SoSe 23
Polymer type
Adjusting the polymer concentration
Molecular weight
Most hydrogels that are used in biofabrication and 3D pioprinting applications exhibit a non-newtonioan flow behaviour. Which mathematical models can be used to describe the flow behaviour of these.
Ostwald de waele model
Carreau viscosity model
Cross viscosity equation
Last changeda year ago
