Questionary Akis "Ubiquitin and Proteasome"

1. Ub activation

   uibiquitinin is activated by the E1 ibiquitinn-activatng enyzme

2. Conjugation

   activtaed ubiquitinin is transferred from E1 to an E2 ubiquitinin-conjugating enzyme

3. ligation

   ligase brings E2 and the target protein together and transfers biquitinin to the substrate

4. more ubiquitinin can be attached to the chain, which is send to the proteasome

Polyubiquitinated proteins are recognized by the 26S proteasome, unfolded in an ATP-dependent process, translocated into the 20S core, and cleaved into peptides by the catalytic β-subunits

• head

• the 20S core particle

• ATPase ring

• base

• lid